HomeNewsThe Enzymatic Activity of TcPanD

The Enzymatic Activity of TcPanD

Enzymatic activity of TcPanD was determined by mixing 30 mL of purified enzyme, 920 mL of 0.1 M phosphate buffer, and 50 mM l-aspartate. The reaction mixture was adjusted to pH 7.0 using NaOH, incubated at 37 degC for 20 min, and stopped by adding 100 mL of 2 M NaOH. After incubation, samples were derivatized with 2,4-dinitrofluorobenzene, and analyzed by UV absorbance at 360 nm. One unit of TcPanD activity is defined as the enzyme’s ability to degrade 1 mmol b-alanine per minute.

enzymatic activity

The TCPAMD enzyme, also known as tcpaMd, is a key regulator of cell metabolism. Its role is to reduce the levels of a compound known as 3-hydroxypropanoate (THP), a neurotoxic metabolite. To understand how the enzyme operates, its sequence is described in this statement. The protein is encoded by the genes aat, ala, and alt, which are all positioned at the cis-aconitic acid decarboxylase locus.

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